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deaD [2018-01-31 17:16:18]
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deaD [2018-01-31 17:16:18]

DEAD-box RNA helicase
Locus
BSU39110
Isoelectric point
7.71
Molecular weight
53.85 kDa
Protein length
479 aa Sequence Blast
Gene length
1437 bp Sequence Blast
Function
RNA helicase
Product
DEAD-box RNA helicase
Essential
no
Synonyms
yxiN
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → RNA synthesis and degradation → DEAD-box RNA helicases
  • Information processing → Protein synthesis, modification and degradation → Translation → Translation/ other

    • Gene

    Coordinates
    4,015,987 → 4,017,426

    The protein

    Catalyzed reaction/ biological activity

  • RNA helicase

    • Protein family

  • DEAD-box RNA helicases

    • Paralogous protein(s)

  • CshA, CshB, YfmL

    • Domains

  • Helicase domain 1: AS 1-203
  • Linker region: AS 204-212
  • Helicase domain 2: AS 207-368
  • RNA-binding domain: AS 404-479

    • Structure

  • 2G0C (RNA-binding domain, AA 404-479), 2HJV (second domain, AA 207-368); 3MOJ (RNA binding domain complexed with a fragment of 23S ribosomal RNA) PubMed

    • Expression and Regulation

    Operons

    Genes
    yxiM-deaD
    Description
    PubMed

    Regulation

  • expression declines in the stationary phase PubMed
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    Biological materials

    Mutant

  • MGNA-B720 (deaD::erm), available at the NBRP B. subtilis, Japan
  • GP1052 (ΔdeaD::tet), available in Jörg Stülke's lab
  • BKE39110 (ΔdeaD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTATAAACTCCTGTCTC, downstream forward: _UP4_AAATGATGAATGACCTGCTC
  • BKK39110 (ΔdeaD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTATAAACTCCTGTCTC, downstream forward: _UP4_AAATGATGAATGACCTGCTC

    • Expression vector

  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1065 (spc), available in Jörg Stülke's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • FLAG-tag construct

  • GP1068 (spc, based on pGP1331), available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Dagmar Klostermeier, Biozentrum Basel, Switzerland homepage

    • References

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